Ruby Yu Email & Phone Number | Advisor: Danesh Moazed Genetic and genomic studies of the requirements for siRNA-mediated heterochromatin formation in the fission yeast Schizosaccharomyces pombe. u2022 Made genetic manipulations to successfully identify transcriptional 3u2019 end processing signals as inhibitors of siRNA-mediated heterochromatic silencing u2022 Designed fusion protein and CRISPR-based systems to test whether transcript-chromatin association affects the ability of RNAi to target a genetic locus u2022 Probed epigenetic state and chromatin-bound proteins or nucleic acids using chromatin immunoprecipitation or RNA immunoprecipitation and reverse-transcription followed by quantitative PCR u2022 Designed and modified protocols for construction of multiplexed small RNA sequencing libraries in order to compare small RNA populations and extent of small RNA spreading u2022 Generated novel Python scripts to characterize ChIP-seq, siRNA-seq, and RNA-seq data and compare libraries to identify loci of interest u2022 Created lab-wide resources for NGS library analyses.

Ruby Yu

Advisor: Danesh Moazed Genetic and genomic studies of the requirements for siRNA-mediated heterochro...
United States

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Ruby Yu’s Email ruby.yu@ucsf.edu

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Ruby Yu’s Location United States

Ruby Yu’s Current Industry University Of California San Francisco

Ruby Yu’s Prior Industry Harvard University

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Postdoctoral Fellow

2017-02-01 00:00:00 - Present

Postdoctoral Fellow

2016-06-01 00:00:00 - 2017-01-01 00:00:00

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Ruby Yu is a Advisor: Danesh Moazed Genetic and genomic studies of the requirements for siRNA-mediated heterochromatin formation in the fission yeast Schizosaccharomyces pombe. u2022 Made genetic manipulations to successfully identify transcriptional 3u2019 end processing signals as inhibitors of siRNA-mediated heterochromatic silencing u2022 Designed fusion protein and CRISPR-based systems to test whether transcript-chromatin association affects the ability of RNAi to target a genetic locus u2022 Probed epigenetic state and chromatin-bound proteins or nucleic acids using chromatin immunoprecipitation or RNA immunoprecipitation and reverse-transcription followed by quantitative PCR u2022 Designed and modified protocols for construction of multiplexed small RNA sequencing libraries in order to compare small RNA populations and extent of small RNA spreading u2022 Generated novel Python scripts to characterize ChIP-seq, siRNA-seq, and RNA-seq data and compare libraries to identify loci of interest u2022 Created lab-wide resources for NGS library analyses.